STAMBPL1 Antibody

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ProSci
Product Code: PSI-61-882
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-882-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the detected STAMBPL1 protein (arrow).
3 / 4
4 / 4
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the detected STAMBPL1 protein (arrow).
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
AMSH-like protease, AMSH-LP, 3419-, STAM-binding protein-like 1, STAMBPL1, AMSHLP, KIAA1373
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
STAMBPL1 is zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains.
Background References:
  • Reyes-Ibarra, A.P., et al. Mol. Endocrinol. 21(6):1394-1407(2007)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Nakamura, M., et al. Genes Cells 11(6):593-606(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This STAMBPL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human STAMBPL1.
NCBI Gene ID #:
57559
NCBI Official Name:
AMSH-like protease
NCBI Official Symbol:
STAMBPL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q96FJ0
Protein GI Number:
71153542
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q96FJ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.