ALKBH6 Antibody

ProSci
Product Code: PSI-55-757
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-757-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 5
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
3 / 5
4 / 5
ALKBH6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
5 / 5
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
ALKBH6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6, 11411-, Alkylated DNA repair protein alkB homolog 6, ALKBH6, ABH6
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).
Background References:
  • Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ALKBH6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the Central region of human ALKBH6.
NCBI Gene ID #:
84964
NCBI Official Name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6
NCBI Official Symbol:
ALKBH6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
Q3KRA9
Protein GI Number:
172046713
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q3KRA9
User NOte:
Optimal dilutions for each application to be determined by the researcher.