FBXW8 Antibody

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ProSci
Product Code: PSI-55-367
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-367-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
F-box/WD repeat-containing protein 8, F-box and WD-40 domain-containing protein 8, F-box only protein 29, FBXW8, FBW6, FBW8, FBX29, FBXO29, FBXW6
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
Background References:
  • Tsutsumi, T., et al. Mol. Cell. Biol. 28(2):743-751(2008)
  • Koch, H.B., et al. Cell Cycle 6(2):205-217(2007)
  • Okabe, H., et al. PLoS ONE 1, E128 (2006) :
  • Watanabe, N., et al. Proc. Natl. Acad. Sci. U.S.A. 101(13):4419-4424(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FBXW8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-299 amino acids from the Central region of human FBXW8.
NCBI Gene ID #:
26259
NCBI Official Name:
F-box/WD repeat-containing protein 8
NCBI Official Symbol:
FBXW8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
67 kDa
Protein Accession #:
Q8N3Y1
Protein GI Number:
296434513
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q8N3Y1
User NOte:
Optimal dilutions for each application to be determined by the researcher.