FBXL5 Antibody

ProSci
Product Code: PSI-63-931
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-931-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of FBXL5 Antibody in HepG2 cell line lysates (35ug/lane).
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Western blot analysis of FBXL5 Antibody in mouse heart tissue lysates (35ug/lane)
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Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of FBXL5 Antibody in HepG2 cell line lysates (35ug/lane).
Western blot analysis of FBXL5 Antibody in mouse heart tissue lysates (35ug/lane)
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
F-box/LRR-repeat protein 5, F-box and leucine-rich repeat protein 5, F-box protein FBL4/FBL5, p45SKP2-like protein, FBXL5, FBL4, FBL5, FLR1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background References:
  • Salahudeen, A.A., et al. Science 326(5953):722-726(2009)
  • Vashisht, A.A., et al. Science 326(5953):718-721(2009)
  • Zhang, N., et al. Biochem. Biophys. Res. Commun. 359(1):34-39(2007)
  • Ilyin, G.P., et al. Genomics 67(1):40-47(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the N-terminal region of human FBXL5.
NCBI Gene ID #:
26234
NCBI Official Name:
F-box/LRR-repeat protein 5
NCBI Official Symbol:
FBXL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
79 kDa
Protein Accession #:
Q9UKA1
Protein GI Number:
37537864
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9UKA1
User NOte:
Optimal dilutions for each application to be determined by the researcher.