CL029 Antibody

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ProSci
Product Code: PSI-55-673
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-673-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Confocal immunofluorescent analysis of CL029 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Confocal immunofluorescent analysis of CL029 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Uncharacterized protein C12orf29, C12orf29
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
May be required for endoplasmic reticulum organization (By similarity).
Background References:
  • Mehrle, A., et al. Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006) :
  • Wiemann, S., et al. Genome Res. 14 (10B), 2136-2144 (2004) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CL029 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-89 amino acids from the N-terminal region of human CL029.
NCBI Gene ID #:
91298
NCBI Official Name:
Uncharacterized protein C12orf29
NCBI Official Symbol:
C12orf29
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
Q8N999
Protein GI Number:
158706467
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q8N999
User NOte:
Optimal dilutions for each application to be determined by the researcher.