Mouse Map3k5 Antibody

ProSci
Product Code: PSI-57-737
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-737-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of Map3k5 using rabbit polyclonal Mouse Map3k5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k5 gene.
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Western blot analysis of Map3k5 using rabbit polyclonal Mouse Map3k5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k5 gene.
Western blot analysis in mouse bladder tissue lysates (35ug/lane).

Further Information

Additional Names:
Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, Map3k5, Ask1, Mekk5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death (By similarity).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Map3k5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1175-1203 amino acids from the C-terminal region of mouse Map3k5.
NCBI Gene ID #:
26408
NCBI Official Name:
Mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol:
Map3k5
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
155 kDa
Protein Accession #:
O35099
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,
Swissprot #:
O35099
User NOte:
Optimal dilutions for each application to be determined by the researcher.