Mouse Wee2 Antibody

ProSci
Product Code: PSI-57-755
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-755-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HepG2 cell line lysates (35ug/lane).
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Mouse Wee2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse ovarian tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in HepG2 cell line lysates (35ug/lane).
Mouse Wee2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse ovarian tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Wee1-like protein kinase 2, Wee1-like protein kinase 1B, Wee1B kinase, mWee1B, Wee2, Wee1b
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Phosphorylates and inhibits CDK1. May act as a negative regulator of entry into mitosis (G2 to M transition).
Background References:
  • Oh, J.S., et al. J. Cell Biol. 188(2):199-207(2010)
  • Joshi, S., et al. BMC Dev. Biol. 7, 67 (2007) :
  • Han, S.J., et al. Curr. Biol. 15(18):1670-1676(2005)
  • Nakanishi, M., et al. Genes Cells 5(10):839-847(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Wee2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from the Central region of mouse Wee2.
NCBI Gene ID #:
381759
NCBI Official Name:
Wee1-like protein kinase 2
NCBI Official Symbol:
Wee2
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
Q66JT0
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Cell Cycle,Signal Transduction
Swissprot #:
Q66JT0
User NOte:
Optimal dilutions for each application to be determined by the researcher.