TPPP3 Antibody

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ProSci
Product Code: PSI-61-751
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-751-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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(LEFT)Western blot analysis of TPPP3 Antibody in WiDr cell tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of TPPP3 Antibody in mouse cerebellum tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.
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TPPP3 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.
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(LEFT)Western blot analysis of TPPP3 Antibody in WiDr cell tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of TPPP3 Antibody in mouse cerebellum tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.
TPPP3 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Tubulin polymerization-promoting protein family member 3, TPPP/p20, TPPP3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
TPPP3 binds tubulin and has microtubule bundling activity. TPPP3 may play a role in cell proliferation and mitosis.
Background References:
  • Zhou, W., et al. Mol. Cell. Biochem. 333 (1-2), 91-98 (2010) Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009) Vincze, O., et al. Biochemistry 45(46):13818-13826(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This TPPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human TPPP3.
NCBI Gene ID #:
51673
NCBI Official Name:
Tubulin polymerization-promoting protein family member 3
NCBI Official Symbol:
TPPP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
Q9BW30
Protein GI Number:
28380040
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Cell Cycle,Signal Transduction
Swissprot #:
Q9BW30
User NOte:
Optimal dilutions for each application to be determined by the researcher.