CA3 Antibody

ProSci
Product Code: PSI-63-077
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-077-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CA3 Antibody in mouse liver tissue lysates (35ug/lane)
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CA3 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of CA3 Antibody in mouse liver tissue lysates (35ug/lane)
CA3 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Carbonic anhydrase 3, Carbonate dehydratase III, Carbonic anhydrase III, CA-III, CA3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Carbonic anhydrase III (CAIII) is a member of carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.
Background References:
  • Du,A.L., Autoimmunity 42 (3), 209-215 (2009)
  • Dai,H.Y., Mol. Carcinog. 47 (12), 956-963 (2008)
  • Gailly,P., Kidney Int. 74 (1), 52-61 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-177 amino acids from the Central region of human CA3.
NCBI Gene ID #:
761
NCBI Official Name:
Carbonic anhydrase 3
NCBI Official Symbol:
CA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
P07451
Protein GI Number:
134047703
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Immunology
Swissprot #:
P07451
User NOte:
Optimal dilutions for each application to be determined by the researcher.