NEDD4 Antibody

ProSci
Product Code: PSI-64-194
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-194-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis of NEDD4 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
2 / 3
NEDD4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis of NEDD4 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
NEDD4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
E3 ubiquitin-protein ligase NEDD4, 632-, Cell proliferation-inducing gene 53 protein, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4, NEDD4, KIAA0093, NEDD4-1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Nedd4 is one of a group of mouse genes that show developmentally regulated expression in mouse embryonic brain. Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.
Background References:
  • Lin, Q., et al. Mol. Cell. Biol. 30(6):1541-1554(2010)
  • Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
  • Edwin, F., et al. J. Biol. Chem. 285(1):255-264(2010)
  • Guo, Y.Y., et al. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 31(6):679-685(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This NEDD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1288-1319 amino acids from the C-terminal region of human NEDD4.
NCBI Gene ID #:
4734
NCBI Official Name:
E3 ubiquitin-protein ligase NEDD4
NCBI Official Symbol:
NEDD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
149 kDa
Protein Accession #:
P46934
Protein GI Number:
313104311
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Neuroscience
Swissprot #:
P46934
User NOte:
Optimal dilutions for each application to be determined by the researcher.