HIP2 Antibody

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ProSci
Product Code: PSI-61-078
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-078-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody . Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 3
Fluorescent image of Hela cell stained with HIP2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). HIP2 immunoreactivity is localized to Cytoplasm significantly.
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Immunohistochemical analysis of paraffin-embedded H. small intestine section using HIP2 Antibody . Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cell stained with HIP2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). HIP2 immunoreactivity is localized to Cytoplasm significantly.

Documents

Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 K, Huntingtin-interacting protein 2, HIP-2, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2-25 kDa, Ubiquitin-conjugating enzyme E2(25K), Ubiquitin-conjugating enzyme E2-25K, Ubiquitin-protein ligase, UBE2K, HIP2, LIG
Application Note:
For IHC-P starting dilution is: 1:100

For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.
Background References:
  • Furukawa, Y., et al., Electrophoresis 21(2):338-346 (2000).
  • Kikuchi, J., et al., Arterioscler. Thromb. Vasc. Biol. 20(1):128-134 (2000).
  • Petersen, A., et al., Exp. Neurol. 157(1):1-18 (1999).
  • Kalchman, M.A., et al., J. Biol. Chem. 271(32):19385-19394 (1996).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This HIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-139 amino acids from the Central region of human HIP2.
NCBI Gene ID #:
3093
NCBI Official Name:
Ubiquitin-conjugating enzyme E2 K
NCBI Official Symbol:
UBE2K
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
P61086
Protein GI Number:
46577658
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Neuroscience
Swissprot #:
P61086
User NOte:
Optimal dilutions for each application to be determined by the researcher.