MSX1 Antibody

Contact us
ProSci
Product Code: PSI-60-419
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-419-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Western Blot at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 ug per lane.
2 / 5
Fluorescent image of U251 cell stained with MSX1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).MSX1 immunoreactivity is localized to Nucleus significantly.
3 / 5
4 / 5
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
5 / 5
Fluorescent confocal image of SY5Y cells stained with MSX1 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MSX1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.
❮❯

Western Blot at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 ug per lane.
Fluorescent image of U251 cell stained with MSX1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).MSX1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
Fluorescent confocal image of SY5Y cells stained with MSX1 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MSX1 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.

Documents

Further Information

Additional Names:
Homeobox protein MSX-1, Homeobox protein Hox-7, Msh homeobox 1-like protein, MSX1, HOX7
Application Note:
For WB starting dilution is: 1:2000

For IF starting dilution is: 1:10~50
Background:
This gene encodes a member of the muscle segment homeobox gene family. The encoded protein functions as a transcriptional repressor during embryogenesis through interactions with components of the core transcription complex and other homeoproteins. It may also have roles in limb-pattern formation, craniofacial development, particularly odontogenesis, and tumor growth inhibition. Mutations in this gene, which was once known as homeobox 7, have been associated with nonsyndromic cleft lip with or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome, and autosomoal dominant hypodontia.
Background References:
  • References for protein:
  • Sliwinski, T., et al. Cancer Epidemiol 34(5):652-655(2010)
  • Nikopensius, T., et al. Birth Defects Res. PartClin. Mol. Teratol. 88(9):748-756(2010)
  • Suazo, J., et al. Am. J. Med. Genet.152A (8), 2011-2016 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MSX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human MSX1.
NCBI Gene ID #:
4487
NCBI Official Name:
Homeobox protein MSX-1
NCBI Official Symbol:
MSX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
P28360
Protein GI Number:
557952603
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P28360
User NOte:
Optimal dilutions for each application to be determined by the researcher.