FLI1 Antibody

ProSci
Product Code: PSI-62-290
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-290-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of FLI1 antibody in mouse lung tissue lysates(35ug/lane)
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Confocal immunofluorescent analysis of FLI1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Western blot analysis of FLI1 antibody in mouse lung tissue lysates(35ug/lane)
Confocal immunofluorescent analysis of FLI1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Further Information

Additional Names:
Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
FLI1 is a sequence-specific transcriptional activator. It recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Background References:
  • Embree,L.J., Cancer Res. 69 (10), 4363-4371 (2009)
  • Torlakovic,E.E., Histol. Histopathol. 23 (11), 1309-1314 (2008)
  • Cironi,L., PLoS ONE(7), E2634 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Drosophila, Zebrafish, Rat, Bovine, Xenopus
Immunogen:
This FLI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-328 amino acids from the Central region of human FLI1.
NCBI Gene ID #:
2313
NCBI Official Name:
Friend leukemia integration 1 transcription factor
NCBI Official Symbol:
FLI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q01543
Protein GI Number:
399496
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity
Swissprot #:
Q01543
User NOte:
Optimal dilutions for each application to be determined by the researcher.