IDE Antibody

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ProSci
Product Code: PSI-57-818
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-818-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A375 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse liver lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of lysate from K562 cell line, using IDE Antibody at 1:1000.
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A375 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse liver lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from K562 cell line, using IDE Antibody at 1:1000.

Documents

Further Information

Additional Names:
Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:10~50
Background:
IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
Background References:
  • Vepsalainen,S.,J. Med. Genet. 44 (9), 606-608 (2007)
  • Kim,M.,J. Biol. Chem. 282 (11), 7825-7832 (2007)
  • Radulescu,R.T.,Int. J. Oncol. 30 (1), 73-80 (2007)
  • Li,Q.,Cell 127 (2), 305-316 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Bovine
Immunogen:
This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 amino acids from the Central region of human IDE.
NCBI Gene ID #:
3416
NCBI Official Name:
Insulin-degrading enzyme
NCBI Official Symbol:
IDE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
118 kDa
Protein Accession #:
P14735
Protein GI Number:
215274252
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P14735
User NOte:
Optimal dilutions for each application to be determined by the researcher.