CYC1 Antibody

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ProSci
Product Code: PSI-55-969
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-969-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).
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Western blot analysis in mouse brain tissue lysates (35ug/lane).
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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in mouse brain tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Background References:
  • Johnatty, S.E., et al. PLoS Genet.(7), E1001016 (2010) :
  • Cummings, C., et al. Apoptosis 11(7):1121-1129(2006)
  • Wen, J.J., et al. Free Radic. Biol. Med. 37(12):2072-2081(2004)
  • An, J., et al. J. Biol. Chem. 279(18):19133-19140(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-291 amino acids from the C-terminal region of human CYC1.
NCBI Gene ID #:
1537
NCBI Official Name:
Cytochrome c1, heme protein, mitochondrial
NCBI Official Symbol:
CYC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
P08574
Protein GI Number:
311033458
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P08574
User NOte:
Optimal dilutions for each application to be determined by the researcher.