DDIT3 Antibody
Code | Size | Price |
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PSI-56-139-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP zeta, C/EBP-homologous protein, CHOP, C/EBP-homologous protein 10, CHOP-10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153, DDIT3, CHOP, CHOP10, GADD153
Application Note:
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:2000
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:2000
For IF starting dilution is: 1:10~50
Background:
This gene encodes a member of the CCAAT/enhancer-binding
protein (C/EBP) family of transcription factors. The protein
functions as a dominant-negative inhibitor by forming heterodimers
with other C/EBP members, such as C/EBP and LAP (liver activator
protein), and preventing their DNA binding activity. The protein is
implicated in adipogenesis and erythropoiesis, is activated by
endoplasmic reticulum stress, and promotes apoptosis. Fusion of
this gene and FUS on chromosome 16 or EWSR1 on chromosome 22
induced by translocation generates chimeric proteins in myxoid
liposarcomas or Ewing sarcoma. Multiple alternatively spliced
transcript variants encoding two isoforms with different length
have been identified.
Background References:
- Park, S.H., et al. J. Immunol. 185(9):5522-5530(2010)
- Goodall, J.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(41):17698-17703(2010)
- Zhang, H.M., et al. J. Virol. 84(17):8446-8459(2010)
- Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Hamster, Bovine
Immunogen:
This DDIT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-149 amino acids from the C-terminal region of human DDIT3.
NCBI Gene ID #:
1649
NCBI Official Name:
DNA damage-inducible transcript 3 protein
NCBI Official Symbol:
DDIT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
P35638
Protein GI Number:
544022
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P35638
User NOte:
Optimal dilutions for each application to be determined by the researcher.