UGT8 antibody

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ProSci
Product Code: PSI-55-954
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-954-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + human heart lysates Lysates/proteins at 20 ug per lane.
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UGT8 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western Blot at 1:2000 dilution + mouse cerebellum lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human heart lysates Lysates/proteins at 20 ug per lane.
UGT8 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase, Ceramide UDP-galactosyltransferase, Cerebroside synthase, UDP-galactose-ceramide galactosyltransferase, UGT8, CGT, UGT4
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:10~50
Background:
Galactocerebrosides are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system and are also present in small amounts in kidney. The key enzymatic step in the biosynthesis of galactocerebrosides consists of the transfer of galactose to ceramide catalyzed by UDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45). The enzyme encoded by the CGT gene is the first involved in complex lipid biosynthesis in the myelinating oligodendrocyte.[supplied by OMIM].
Background References:
  • Dziecedil Giel, P., et al. Br. J. Cancer 103(4):524-531(2010)
  • Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
  • Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
  • Ruckhaberle, E., et al. J. Cancer Res. Clin. Oncol. 135(8):1005-1013(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This UGT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the Central region of human UGT8.
NCBI Gene ID #:
7368
NCBI Official Name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
NCBI Official Symbol:
UGT8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q16880
Protein GI Number:
296434442
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q16880
User NOte:
Optimal dilutions for each application to be determined by the researcher.