ETHE1 Antibody

Contact us
ProSci
Product Code: PSI-62-374
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-374-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis of ETHE1 Antibody in mouse liver tissue lysates (35ug/lane)
2 / 2
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ETHE1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of 293 cells using ETHE1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
❮❯

Western blot analysis of ETHE1 Antibody in mouse liver tissue lysates (35ug/lane)
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ETHE1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of 293 cells using ETHE1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Persulfide dioxygenase ETHE1, mitochondrial, Ethylmalonic encephalopathy protein 1, Hepatoma subtracted clone one protein, Sulfur dioxygenase ETHE1, ETHE1, HSCO
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
ETHE1 is a sulfur dioxygenase that localizes within the mitochondrial matrix. The enzyme functions in sulfide catabolism. Mutations in its gene result in ethylmalonic encephalopathy.
Background References:
  • Tiranti,V., Nat. Med. 15 (2), 200-205 (2009)
  • Mineri,R., J. Med. Genet. 45 (7), 473-478 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ETHE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-209 amino acids from the C-terminal region of human ETHE1.
NCBI Gene ID #:
23474
NCBI Official Name:
Persulfide dioxygenase ETHE1, mitochondrial
NCBI Official Symbol:
ETHE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
O95571
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
O95571
User NOte:
Optimal dilutions for each application to be determined by the researcher.