CYC1 Antibody

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ProSci
Product Code: PSI-61-032
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-032-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent image of Hela cells stained with CYC1 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
2 / 5
Immunohistochemical analysis of paraffin-embedded H. stomach section using CYC1 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
3 / 5
4 / 5
Immunohistochemical analysis of paraffin-embedded R. stomach section using CYC1 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
5 / 5
Western blot analysis of lysates from A431, Hela, HepG2, MCF-7, U-251 MG cell lines and mouse brain tissue lysate (from left to right), using CYC1 Antibody at 1:1000 at each lane.
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Fluorescent image of Hela cells stained with CYC1 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. stomach section using CYC1 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded R. stomach section using CYC1 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from A431, Hela, HepG2, MCF-7, U-251 MG cell lines and mouse brain tissue lysate (from left to right), using CYC1 Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1
Application Note:
For IF starting dilution is: 1:25

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Background References:
  • Nishikimi M.,et al.Nucleic Acids Res. 16:3577-3577(1988).
  • Suzuki H.,et al.J. Biol. Chem. 264:1368-1374(1989).
  • Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
  • Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CYC1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 142-176 amino acids from the Central region of human CYC1.
NCBI Gene ID #:
1537
NCBI Official Name:
Cytochrome c1, heme protein, mitochondrial
NCBI Official Symbol:
CYC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
P08574
Protein GI Number:
311033458
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P08574
User NOte:
Optimal dilutions for each application to be determined by the researcher.