TBX6 Antibody

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ProSci
Product Code: PSI-56-208
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-208-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).
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Fluorescent confocal image of Hela cell stained with TBX6 Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.
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Western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with TBX6 Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.

Documents

Further Information

Additional Names:
T-box transcription factor TBX6, T-box protein 6, TBX6
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures.
Background References:
  • Fei, Q., et al. Spine 35(9):983-988(2010)
  • Ghebranious, N., et al. J. Bone Miner. Res. 23(10):1576-1583(2008)
  • Farin, H.F., et al. J. Biol. Chem. 282(35):25748-25759(2007)
  • Papapetrou, C., et al. Genomics 55(2):238-241(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This TBX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the Central region of human TBX6.
NCBI Gene ID #:
6911
NCBI Official Name:
T-box transcription factor TBX6
NCBI Official Symbol:
TBX6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
O95947
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
O95947
User NOte:
Optimal dilutions for each application to be determined by the researcher.