GAK Antibody

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ProSci
Product Code: PSI-63-372
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-372-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of GAK using GAK Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis in mouse brain tissue lysates (35ug/lane).
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Western blot analysis of GAK using GAK Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis in mouse brain tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Cyclin-G-associated kinase, GAK
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. GAK localizes to the perinuclear area and to the trans-Golgi network. It is also observed on the plasma membrane, probably at focals adhesions. Expression is ubiquitous, wiht highest levels in testis. The protein contains 1 J domain and 1 tensin domain.
Background References:
  • Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
  • Greener, T., et al., J. Biol. Chem. 275(2):1365-1370 (2000).
  • Kimura, S.H., et al., Genomics 44(2):179-187 (1997).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This GAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1140-1170 amino acids from the C-terminal region of human GAK.
NCBI Gene ID #:
2580
NCBI Official Name:
Cyclin-G-associated kinase
NCBI Official Symbol:
GAK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
143 kDa
Protein Accession #:
O14976
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
O14976
User NOte:
Optimal dilutions for each application to be determined by the researcher.