S100B Antibody

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ProSci
Product Code: PSI-61-780
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-780-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from A431 cell line, mouse brain, rat brain tissue (from left to right), using S100B Antibody at 1:1000 at each lane.
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S100B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human malignant melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysates from A431 cell line, mouse brain, rat brain tissue (from left to right), using S100B Antibody at 1:1000 at each lane.
S100B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human malignant melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Background References:
  • Davila, S., et al. Genes Immun. 11(3):232-238(2010) Mori, T., et al. Glia 58(3):300-314(2010) Steiner, J., et al. Psychoneuroendocrinology 35(2):321-324(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This S100B antibody is generated from rabbits immunized with S100B recombinant protein.
NCBI Gene ID #:
6285
NCBI Official Name:
Protein S100-B
NCBI Official Symbol:
S100B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
11 kDa
Protein Accession #:
P04271
Protein GI Number:
134138
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P04271
User NOte:
Optimal dilutions for each application to be determined by the researcher.