RAN Antibody
| Code | Size | Price |
|---|
| PSI-62-407-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
RAN (ras-related nuclear protein) is a small GTP binding
protein belonging to the RAS superfamily that is essential for the
translocation of RNA and proteins through the nuclear pore complex.
The RAN protein is also involved in control of DNA synthesis and
cell cycle progression. Nuclear localization of RAN requires the
presence of regulator of chromosome condensation 1 (RCC1).
Mutations in RAN disrupt DNA synthesis. Because of its many
functions, it is likely that RAN interacts with several other
proteins. RAN regulates formation and organization of the
microtubule network independently of its role in the
nucleus-cytosol exchange of macromolecules. RAN could be a key
signaling molecule regulating microtubule polymerization during
mitosis. RCC1 generates a high local concentration of RAN-GTP
around chromatin which, in turn, induces the local nucleation of
microtubules. RAN is an androgen receptor (AR) coactivator that
binds differentially with different lengths of polyglutamine within
the androgen receptor. Polyglutamine repeat expansion in the AR is
linked to Kennedy's disease (X-linked spinal and bulbar muscular
atrophy). RAN coactivation of the AR diminishes with polyglutamine
expansion within the AR, and this weak coactivation may lead to
partial androgen insensitivity during the development of Kennedy's
disease.
Background References:
- Kim, J.S., et al. Mol. Carcinog. 49(10):913-921(2010)
- Liu, C., et al. Mol. Cell. Biol. 30(16):3956-3969(2010)
- Boni, V., et al. Pharmacogenomics J. (2010) In press :
- Ly, T.K., et al. J. Biol. Chem. 285(8):5815-5826(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Zebrafish, Drosophila, Monkey, Rat, Xenopus
Immunogen:
This RAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the Central region of human RAN.
NCBI Gene ID #:
5901
NCBI Official Name:
GTP-binding nuclear protein Ran
NCBI Official Symbol:
RAN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
P62826
Protein GI Number:
51338598
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P62826
User NOte:
Optimal dilutions for each application to be determined by the researcher.