MLF1 Antibody
| Code | Size | Price |
|---|
| PSI-62-425-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. The protein interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. It suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Background References:
- Li,Z.F., J. Neurol. Sci. 264 (1-2), 77-86 (2008)
- Yoneda-Kato,N., EMBO J. 24 (9), 1739-1749 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-244 amino acids from the C-terminal region of human MLF1.
NCBI Gene ID #:
4291
NCBI Official Name:
Myeloid leukemia factor 1
NCBI Official Symbol:
MLF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
P58340
Protein GI Number:
17368170
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P58340
User NOte:
Optimal dilutions for each application to be determined by the researcher.