Cellular Apoptosis Susceptibility Antibody
Code | Size | Price |
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PSI-60-497-400ul | 400ul | £662.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
Exportin-2, Exp2, Cellular apoptosis susceptibility protein, Chromosome segregation 1-like protein, Importin-alpha re-exporter, CSE1L, CAS, XPO2
Application Note:
For IHC-P starting dilution is: 1:100
For IF starting dilution is: 1:100
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:100
For WB starting dilution is: 1:1000
Background:
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.
Background References:
- Goldberg, G.S., et al., J. Biol. Chem. 278(47):46533-46540 (2003).
- Behrens, P., et al., Apoptosis 8(1):39-44 (2003).
- Jiang, M.C., et al., Biochem. Biophys. Res. Commun. 294(4):900-905 (2002).
- Wellmann, A., et al., Int. J. Mol. Med. 7(5):489-494 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Bovine
Immunogen:
This Cellular Apoptosis Susceptibility antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human Cellular Apoptosis Susceptibility.
NCBI Gene ID #:
1434
NCBI Official Name:
Exportin-2
NCBI Official Symbol:
CSE1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
P55060
Protein GI Number:
62297557
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P55060
User NOte:
Optimal dilutions for each application to be determined by the researcher.