GAS1 Antibody

ProSci
Product Code: PSI-56-087
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-087-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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GAS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
GAS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Growth arrest-specific protein 1, GAS-1, GAS1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.
Background References:
  • Ribeiro, L.A., et al. Am. J. Med. Genet.152A (7), 1688-1694 (2010)Johnatty, S.E., et al. PLoS Genet.(7), E1001016 (2010)Rittie, L., et al. Aging Cell 8(6):738-751(2009) Zhao, L., et al. J. Biol. Chem. 284(39):26273-26285(2009) Dominguez-Monzon, G., et al. Int. J. Dev. Neurosci. 27(4):305-313(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-127 amino acids from the N-terminal region of human GAS1.
NCBI Gene ID #:
2619
NCBI Official Name:
Growth arrest-specific protein 1
NCBI Official Symbol:
GAS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
P54826
Protein GI Number:
218512049
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P54826
User NOte:
Optimal dilutions for each application to be determined by the researcher.