AIRE Antibody

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ProSci
Product Code: PSI-55-609
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-609-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
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Confocal immunofluorescent analysis of AIRE Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Confocal immunofluorescent analysis of AIRE Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, APECED protein, AIRE, APECED
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Background References:
  • Cervato, S., et al. Clin. Endocrinol. (Oxf) 73(5):630-636(2010)
  • Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
  • Conteduca, G., et al. Clin. Immunol. 136(1):96-104(2010)
  • Colome, N., et al. J. Proteome Res. 9(5):2600-2609(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AIRE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the Central region of human AIRE.
NCBI Gene ID #:
326
NCBI Official Name:
Autoimmune regulator
NCBI Official Symbol:
AIRE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
O43918
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
O43918
User NOte:
Optimal dilutions for each application to be determined by the researcher.