FOLR2 Antibody

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ProSci
Product Code: PSI-61-763
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-763-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from human placenta tissue, using FOLR2 Antibody at 1:1000.
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Western blot analysis of lysates from MDA-MB-453 cell line, human placenta tissue lysate (from left to right), using FOLR2 Antibody at 1:1000 at each lane.
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Western blot analysis of lysate from human placenta tissue lysate, using FOLR2 Antibody at 1:1000.
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Western blot analysis of lysates from KG-1, MCF-7, MDA-MB-453 cell line (from left to right), using FOLR2 Antibody at 1:1000 at each lane.
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Western blot analysis of lysate from mouse heart tissue lysate, using FOLR2 Antibody at 1:1000.
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FOLR2 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysate from human placenta tissue, using FOLR2 Antibody at 1:1000.
Western blot analysis of lysates from MDA-MB-453 cell line, human placenta tissue lysate (from left to right), using FOLR2 Antibody at 1:1000 at each lane.
Western blot analysis of lysate from human placenta tissue lysate, using FOLR2 Antibody at 1:1000.
Western blot analysis of lysates from KG-1, MCF-7, MDA-MB-453 cell line (from left to right), using FOLR2 Antibody at 1:1000 at each lane.
Western blot analysis of lysate from mouse heart tissue lysate, using FOLR2 Antibody at 1:1000.
FOLR2 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Folate receptor beta, FR-beta, Folate receptor 2, Folate receptor, fetal/placental, Placental folate-binding protein, FBP, FOLR2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
FOLR2 is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.
Background References:
  • Puig-Kroger, A., et al. Cancer Res. 69(24):9395-9403(2009) Boyles, A.L., et al. Genet. Epidemiol. 33(3):247-255(2009) Franke, B., et al. Birth Defects Res. PartClin. Mol. Teratol. 85(3):216-226(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FOLR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human FOLR2.
NCBI Gene ID #:
2350
NCBI Official Name:
Folate receptor beta
NCBI Official Symbol:
FOLR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
P14207
Protein GI Number:
116241366
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Obesity,Signal Transduction
Swissprot #:
P14207
User NOte:
Optimal dilutions for each application to be determined by the researcher.