CHAT Antibody

ProSci
Product Code: PSI-64-014
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-014-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CHAT Antibody in NCI-H460 cell line lysates (35ug/lane)
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Western blot analysis of CHAT Antibody in mouse liver tissue lysates (35ug/lane)
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Western blot analysis of CHAT Antibody in NCI-H460 cell line lysates (35ug/lane)
Western blot analysis of CHAT Antibody in mouse liver tissue lysates (35ug/lane)

Further Information

Additional Names:
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
CHAT is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.
Background References:
  • Shaffer, J.R., et al. Nutr Metab Cardiovasc Dis (2010) In press
  • Beck, M., et al. Cell Tissue Res. 338(1):37-51(2009)
  • Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-128 amino acids from the N-terminal region of human CHAT.
NCBI Gene ID #:
1103
NCBI Official Name:
Choline O-acetyltransferase
NCBI Official Symbol:
CHAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
P28329
Protein GI Number:
281185509
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
P28329
User NOte:
Optimal dilutions for each application to be determined by the researcher.