YY1 Antibody

ProSci
Product Code: PSI-61-409
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-409-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-YY1 Ctr Pab in HL60 cell lysate (lane 1) and in mouse kidney tissue lysate (lane 2)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-YY1 Ctr Pab in HL60 cell lysate (lane 1) and in mouse kidney tissue lysate (lane 2)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, NF-E1, Yin and yang 1, YY-1, YY1, INO80S
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
Background References:
  • Sucharov, C.C., et al., J. Biol. Chem. 278(33):31233-31239 (2003).
  • Kurisaki, K., et al., Mol. Cell. Biol. 23(13):4494-4510 (2003).
  • Huang, N.E., et al., Biochem. Biophys. Res. Commun. 306(1):267-275 (2003).
  • Moriuchi, M., et al., J. Biol. Chem. 278(15):13003-13007 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This YY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the Central region of human YY1.
NCBI Gene ID #:
7528
NCBI Official Name:
Transcriptional repressor protein YY1
NCBI Official Symbol:
YY1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P25490
Protein GI Number:
3915889
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
P25490
User NOte:
Optimal dilutions for each application to be determined by the researcher.