KCNJ2 Antibody

ProSci
Product Code: PSI-62-541
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-541-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of KCNJ2 Antibody in mouse lung tissue lysates (35ug/lane)
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Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of KCNJ2 Antibody in mouse lung tissue lysates (35ug/lane)
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Inward rectifier potassium channel 2, Cardiac inward rectifier potassium channel, Inward rectifier K(+) channel Kir21, IRK-1, hIRK1, Potassium channel, inwardly rectifying subfamily J member 2, KCNJ2, IRK1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. This protein is an integral membrane protein and inward-rectifier type potassium channel. This protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Mutations in this gene have been associated with Andersen syndrome, which is racterized by periodic paralysis, cardiac arrhythmias, and dysmorphic features.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Pig, Rabbit, Rat
Immunogen:
This KCNJ2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-427 amino acids from the C-terminal region of human KCNJ2.
NCBI Gene ID #:
3759
NCBI Official Name:
Inward rectifier potassium channel 2
NCBI Official Symbol:
KCNJ2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P63252
Protein GI Number:
54037433
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
P63252
User NOte:
Optimal dilutions for each application to be determined by the researcher.