GFAP Antibody

ProSci
Product Code: PSI-60-910
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-910-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Antibody is used in Western blot to detect GFAP in mouse brain tissue lysate.
2 / 2
Western blot analysis of GFAP using rabbit polyclonal GFAP Antibody (T396) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GFAP gene.

Antibody is used in Western blot to detect GFAP in mouse brain tissue lysate.
Western blot analysis of GFAP using rabbit polyclonal GFAP Antibody (T396) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GFAP gene.

Further Information

Additional Names:
Glial fibrillary acidic protein, GFAP, GFAP
Application Note:
For WB starting dilution is: 1:1000
Background:
GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.
Background References:
  • Quintanar, J.L., et al., Parasitol. Res. 90(4):261-263 (2003).
  • Shiroma, N., et al., Brain Dev. 25(2):116-121 (2003).
  • Nielsen, A.L., et al., J. Biol. Chem. 277(33):29983-29991 (2002).
  • Namekawa, M., et al., Ann. Neurol. 52(6):779-785 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This GFAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-410 amino acids from the C-terminal region of human GFAP.
NCBI Gene ID #:
2670
NCBI Official Name:
Glial fibrillary acidic protein
NCBI Official Symbol:
GFAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
P14136
Protein GI Number:
121135
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P14136
User NOte:
Optimal dilutions for each application to be determined by the researcher.