DNM2 Antibody

Contact us
ProSci
Product Code: PSI-61-336
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-336-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 2
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: mouse brain lysate Lane 5: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
❮❯

Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: mouse brain lysate Lane 5: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Dynamin-2, 3.6.5.5, DNM2, DYN2
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis.
Background References:
  • Diatloff-Zito C.,et al.Gene 163:301-306(1995).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Grimwood J.,et al.Nature 428:529-535(2004).
  • Okamoto P.M.,et al.J. Biol. Chem. 276:48458-48465(2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This DNM2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 213-247 amino acids from human DNM2.
NCBI Gene ID #:
1785
NCBI Official Name:
Dynamin-2
NCBI Official Symbol:
DNM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
98 kDa
Protein Accession #:
P50570
Protein GI Number:
47117856
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P50570
User NOte:
Optimal dilutions for each application to be determined by the researcher.