KCTD11 Antibody

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ProSci
Product Code: PSI-61-142
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-142-200ul200ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining KCTD11 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:1000-1:2000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: mouse cerebellum lysate Lysates/proteins at 20 ug per lane.
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Antibody staining KCTD11 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:1000-1:2000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: mouse cerebellum lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
BTB/POZ domain-containing protein KCTD11, KCTD11, C17orf36, REN
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000-1:2000
Background:
Plays a role as a marker and a regulator of neuronal differentiation; Up-regulated by a variety of neurogenic signals, such as retinoic acid, epidermal growth factor/EGF and NGFB/nerve growth factor. Induces apoptosis, growth arrest and the expression of cyclin-dependent kinase inhibitor CDKN1B. Plays a role as a tumor repressor and inhibits cell growth and tumorigenicity of medulloblastoma (MDB). Acts as an E3 ubiquitin-protein ligase towards HDAC1, leading to its proteasomal degradation. Functions as antagonist of the Hedgehog pathway on cell proliferation and differentiation by affecting the nuclear transfer of transcrition factor GLI1, thus maintaining cerebellar granule cells in undifferentiated state, this effect probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. When knock- down, Hedgehog antagonism is impaired and proliferation of granule cells is sustained. Activates the caspase cascade.
Background References:
  • Di Marcotullio L.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:10833-10838(2004).
  • Correale S.,et al.Biochimie 93:715-724(2011).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KCTD11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-53 amino acids from human KCTD11.
NCBI Gene ID #:
147040
NCBI Official Name:
BTB/POZ domain-containing protein KCTD11
NCBI Official Symbol:
KCTD11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
Q693B1
Protein GI Number:
74708977
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q693B1
User NOte:
Optimal dilutions for each application to be determined by the researcher.