ROR1 Antibody

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ProSci
Product Code: PSI-63-129
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-129-200ul200ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Antibody staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Immunohistochemical analysis of paraffin-embedded H. heart section using ROR1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Western blot analysis of lysates from K562 cell line, human lung, mouse kidney, mouse heart tissue (from left to right), using ROR1 Antibody at 1:1000 at each lane.
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Immunohistochemical analysis of paraffin-embedded H. heart section using ROR1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from K562 cell line, human lung, mouse kidney, mouse heart tissue (from left to right), using ROR1 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the ROR1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1, ROR1, NTRKR1
Application Note:
For FACS starting dilution is: 1:25

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.
Background References:
  • Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001).
  • Reddy, U.R., et al., Genomics 41(2):283-285 (1997).
  • Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996).
  • Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).
NCBI Gene ID #:
4919
NCBI Official Name:
Tyrosine-protein kinase transmembrane receptor ROR1
NCBI Official Symbol:
ROR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
104 kDa
Protein Accession #:
Q01973
Protein GI Number:
118572711
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q01973
User NOte:
Optimal dilutions for each application to be determined by the researcher.