UCK Antibody

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ProSci
Product Code: PSI-63-410
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-410-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect UCK in mouse cerebellum tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Antibody is used in Western blot to detect UCK in mouse cerebellum tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
UMP-CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 27414 {ECO:0000255|HAMAP-Rule:MF_03172}, Deoxycytidylate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, CK {ECO:0000255|HAMAP-Rule:MF_03172}, dCMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, Nucleoside-diphosphate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 2746 {ECO:0000255|HAMAP-Rule:MF_03172}, Uridine monophosphate/cytidine monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, UMP/CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, UMP/CMPK {ECO:0000255|HAMAP-Rule:MF_03172}, CMPK1 {ECO:0000255|HAMAP-Rule:MF_03172}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.
Background References:
  • Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
  • Hu, R.M., et al., Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548 (2000).
  • Hughes, G.J., et al., Electrophoresis 14(11):1216-1222 (1993).
  • Hochstrasser, D.F., et al., Electrophoresis 13(12):992-1001 (1992).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig, Rat
Immunogen:
This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-48 amino acids from the N-terminal region of human UCK.
NCBI Gene ID #:
51727
NCBI Official Name:
UMP-CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}
NCBI Official Symbol:
CMPK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
P30085
Protein GI Number:
12644008
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity
Swissprot #:
P30085
User NOte:
Optimal dilutions for each application to be determined by the researcher.