MBOAT4 Antibody

ProSci
Product Code: PSI-56-340
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-340-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western Blot at 1:2000 dilution + mouse stomach lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
3 / 4
4 / 4
MBOAT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western Blot at 1:2000 dilution + mouse stomach lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
MBOAT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Ghrelin O-acyltransferase, 231-, Membrane-bound O-acyltransferase domain-containing protein 4, O-acyltransferase domain-containing protein 4, MBOAT4, GOAT, OACT4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
MBOAT4 mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.
Background References:
  • Takahashi, T., et al. J. Biochem. 146(5):675-682(2009) Gomez, R., et al. Arthritis Rheum. 60(6):1704-1709(2009) Gutierrez, J.A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(17):6320-6325(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MBOAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from the Central region of human MBOAT4.
NCBI Gene ID #:
619373
NCBI Official Name:
Ghrelin O-acyltransferase
NCBI Official Symbol:
MBOAT4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q96T53
Protein GI Number:
182676418
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity
Swissprot #:
Q96T53
User NOte:
Optimal dilutions for each application to be determined by the researcher.