POLG Antibody
| Code | Size | Price |
|---|
| PSI-58-118-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
Mitochondrial DNA polymerase is heterotrimeric, consisting
of a homodimer of accessory subunits plus a catalytic subunit. The
protein encoded by this gene is the catalytic subunit of
mitochondrial DNA polymerase. The encoded protein contains a
polyglutamine tract near its N-terminus that may be polymorphic.
Defects in this gene are a cause of progressive external
ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory
ataxic neuropathy dysarthria and ophthalmoparesis (SANDO),
Alpers-Huttenlocher syndrome (AHS), and mitochondrial
neurogastrointestinal encephalopathy syndrome (MNGIE). Two
transcript variants encoding the same protein have been found for
this gene.
Background References:
- Tong, Z.B., et al. Fertil. Steril. 94(7):2932-2934(2010)
- Stewart, J.D., et al. Hepatology 52(5):1791-1796(2010)
- Batabyal, D., et al. J. Biol. Chem. 285(44):34191-34201(2010)
- Wang, W., et al. Nucleic Acids Res. (2010) In press :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG.
NCBI Gene ID #:
5428
NCBI Official Name:
DNA polymerase subunit gamma-1
NCBI Official Symbol:
POLG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
140 kDa
Protein Accession #:
P54098
Protein GI Number:
1706507
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity
Swissprot #:
P54098
User NOte:
Optimal dilutions for each application to be determined by the researcher.