PLA2G1B Antibody

ProSci
Product Code: PSI-61-695
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-695-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PLA2G1B Antibody in mouse spleen tissue lysates (35ug/lane)
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Western blot analysis of PLA2G1B using rabbit polyclonal PLA2G1B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLA2G1B gene.
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Western blot analysis of PLA2G1B Antibody in mouse spleen tissue lysates (35ug/lane)
Western blot analysis of PLA2G1B using rabbit polyclonal PLA2G1B Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLA2G1B gene.

Further Information

Additional Names:
Phospholipase A2, Group IB phospholipase A2, Phosphatidylcholine 2-acylhydrolase 1B, PLA2G1B, PLA2, PLA2A, PPLA2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets
Background References:
  • Xu, W., et al. J. Biol. Chem. 284(24):16659-16666(2009)
  • Han, C., et al. J. Cell. Biochem. 105(2):534-545(2008)
  • Kao, W.T., et al. Lipids Health Dis 7, 20 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PLA2G1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the C-terminal region of human PLA2G1B.
NCBI Gene ID #:
5319
NCBI Official Name:
Phospholipase A2
NCBI Official Symbol:
PLA2G1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa
Protein Accession #:
P04054
Protein GI Number:
129404
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P04054
User NOte:
Optimal dilutions for each application to be determined by the researcher.