CTSA Antibody

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ProSci
Product Code: PSI-55-204
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-204-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. kidney section using CTSA Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 5
Western blot analysis of lysates from A431, Hela, HepG2, HT-1080 cell line and human placenta, kidney tissue lysate (from left to right), using CTSA Antibody at 1:1000 at each lane.
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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CTSA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using CTSA Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from A431, Hela, HepG2, HT-1080 cell line and human placenta, kidney tissue lysate (from left to right), using CTSA Antibody at 1:1000 at each lane.
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
CTSA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, PPCA, Protective protein for beta-galactosidase, Lysosomal protective protein 32 kDa chain, Lysosomal protective protein 20 kDa chain, CTSA, PPGB
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
CTSA encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis.
Background References:
  • Reich, M., et al. Immunol. Lett. 128(2):143-147(2010)
  • Bonten, E.J., et al. J. Biol. Chem. 284(41):28430-28441(2009)
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Tatano, Y., et al. J. Med. Invest. 53 (1-2), 103-112 (2006) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CTSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human CTSA.
NCBI Gene ID #:
5476
NCBI Official Name:
Lysosomal protective protein
NCBI Official Symbol:
CTSA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
P10619
Protein GI Number:
20178316
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P10619
User NOte:
Optimal dilutions for each application to be determined by the researcher.