AK3 Antibody

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ProSci
Product Code: PSI-63-413
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-413-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect AK3 in mouse kidney tissue lysate.
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Western blot analysis of AK3 using rabbit polyclonal AK3 Antibody (C-term H38) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AK3 gene (Lane 2).
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Antibody is used in Western blot to detect AK3 in mouse kidney tissue lysate.
Western blot analysis of AK3 using rabbit polyclonal AK3 Antibody (C-term H38) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AK3 gene (Lane 2).

Documents

Further Information

Additional Names:
Adenylate kinase 4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170}, AK 4 {ECO:0000255|HAMAP-Rule:MF_03170}, 27410 {ECO:0000255|HAMAP-Rule:MF_03170}, 2746 {ECO:0000255|HAMAP-Rule:MF_03170}, Adenylate kinase 3-like {ECO:0000255|HAMAP-Rule:MF_03170}, GTP:AMP phosphotransferase AK4 {ECO:0000255|HAMAP-Rule:MF_03170}, AK4 {ECO:0000255|HAMAP-Rule:MF_03170}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
AK3 is a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated.
Background References:
  • Van Rompay, A.R., et al., Eur. J. Biochem. 261(2):509-517 (1999).
  • Yoneda, T., et al., Brain Res. Mol. Brain Res. 62(2):187-195 (1998).
  • Xu, G., et al., Genomics 13(3):537-542 (1992).
  • Robson, E.B., et al., Cytogenet. Cell Genet. 32 (1-4), 144-152 (1982).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the C-terminal region of human AK3.
NCBI Gene ID #:
100507855, 205
NCBI Official Name:
Adenylate kinase 4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170}
NCBI Official Symbol:
AK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
P27144
Protein GI Number:
125157
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P27144
User NOte:
Optimal dilutions for each application to be determined by the researcher.