PGM1 Antibody

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ProSci
Product Code: PSI-61-146
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-146-200ul200ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining PGM1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: human liver lysate Lane 5: mouse brain lysate Lane 6: mouse heart lysate Lysates/proteins at 20 ug per lane.
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Antibody staining PGM1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: human liver lysate Lane 5: mouse brain lysate Lane 6: mouse heart lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
This enzyme participates in both the breakdown and synthesis of glucose.
Background References:
  • Whitehouse D.B.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:411-415(1992).
  • Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Gregory S.G.,et al.Nature 441:315-321(2006).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This PGM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 251-282 amino acids from the Central region of human PGM1.
NCBI Gene ID #:
5236
NCBI Official Name:
Phosphoglucomutase-1
NCBI Official Symbol:
PGM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
P36871
Protein GI Number:
585670
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P36871
User NOte:
Optimal dilutions for each application to be determined by the researcher.