PAICS Antibody

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ProSci
Product Code: PSI-56-752
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-752-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Multifunctional protein ADE2, Phosphoribosylaminoimidazole-succinocarboxamide synthase, SAICAR synthetase, Phosphoribosylaminoimidazole carboxylase, AIR carboxylase, AIRC, PAICS, ADE2, AIRC, PAIS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
  • Ikeda, M., et al. Biol. Psychiatry 67(3):263-269(2010)
  • Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken
Immunogen:
This PAICS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the N-terminal region of human PAICS.
NCBI Gene ID #:
10606
NCBI Official Name:
Multifunctional protein ADE2
NCBI Official Symbol:
PAICS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
P22234
Protein GI Number:
131628
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P22234
User NOte:
Optimal dilutions for each application to be determined by the researcher.