PGD Antibody

Contact us
ProSci
Product Code: PSI-61-935
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-935-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis in 293,MCF-7 cell line lysates (35ug/lane).This demonstrates the detected PGD protein (arrow).
2 / 4
Western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the detected PGD protein (arrow).
3 / 4
4 / 4
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
❮❯

Western blot analysis in 293,MCF-7 cell line lysates (35ug/lane).This demonstrates the detected PGD protein (arrow).
Western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the detected PGD protein (arrow).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
6-phosphogluconate dehydrogenase, decarboxylating, PGD, PGDH
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.
Background References:
  • Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
  • Kerimov, B.F. Vopr. Med. Khim. 48(5):490-496(2002)
  • Rippa, M., et al. Biochim. Biophys. Acta 1429(1):83-92(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Rat
Immunogen:
This PGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the Central region of human PGD.
NCBI Gene ID #:
5226
NCBI Official Name:
6-phosphogluconate dehydrogenase, decarboxylating
NCBI Official Symbol:
PGD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
P52209
Protein GI Number:
20981679
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P52209
User NOte:
Optimal dilutions for each application to be determined by the researcher.