KMO Antibody

ProSci
Product Code: PSI-63-576
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-576-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot analysis of KMO Antibody in CEM cell line lysates (35ug/lane)
3 / 4
4 / 4
Flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of KMO Antibody in CEM cell line lysates (35ug/lane)
Flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Kynurenine 3-monooxygenase {ECO:0000255|HAMAP-Rule:MF_03018}, 114139 {ECO:0000255|HAMAP-Rule:MF_03018}, Kynurenine 3-hydroxylase {ECO:0000255|HAMAP-Rule:MF_03018}, KMO
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Kynurenine 3-monooxygenase (KMO; EC 1.14.13.9) is an NADPH-dependent flavin monooxygenase that catalyzes the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine.
Background References:
  • Ligam,P., et.al., Placenta 26 (6), 498-504 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KMO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-182 amino acids from the Central region of human KMO.
NCBI Gene ID #:
8564
NCBI Official Name:
Kynurenine 3-monooxygenase {ECO:0000255|HAMAP-Rule:MF_03018}
NCBI Official Symbol:
KMO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
O15229
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
O15229
User NOte:
Optimal dilutions for each application to be determined by the researcher.