PISD Antibody

ProSci
Product Code: PSI-63-660
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-660-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PISD Antibody in mouse cerebellum tissue and mouse NIH-3T3 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain reacted with PISD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PISD Antibody in mouse cerebellum tissue and mouse NIH-3T3 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain reacted with PISD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Phosphatidylserine decarboxylase proenzyme, Phosphatidylserine decarboxylase alpha chain, Phosphatidylserine decarboxylase beta chain, PISD
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Phosphatidylserine decarboxylases catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits
Background References:
  • Simpson,J.C., et.al., EMBO Rep.(3), 287-292 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PISD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-250 amino acids from the Central region of human PISD.
NCBI Gene ID #:
23761
NCBI Official Name:
Phosphatidylserine decarboxylase proenzyme
NCBI Official Symbol:
PISD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
Q9UG56
Protein GI Number:
311033492
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9UG56
User NOte:
Optimal dilutions for each application to be determined by the researcher.