PDK4 Antibody

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ProSci
Product Code: PSI-62-612
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-612-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining PDK4 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: human heart lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 ug per lane.
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Fluorescent confocal image of HeLa cells stained with PDK4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PDK4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.
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Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Antibody staining PDK4 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: human heart lysates Lane 2: mouse skeletal muscle lysates Lysates/proteins at 20 ug per lane.
Fluorescent confocal image of HeLa cells stained with PDK4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PDK4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:2000

For IF starting dilution is: 1:100
Background:
PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Background References:
  • References for protein:
  • Rosa, G., et al., Obes. Res. 11(2):176-182 (2003).
  • Razeghi, P., et al., Cardiology 97(4):203-209 (2002).
  • Rowles, J., et al., J. Biol. Chem. 271(37):22376-22382 (1996).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 382-410 amino acids from the C-terminal region of human PDK4.
NCBI Gene ID #:
5166
NCBI Official Name:
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
NCBI Official Symbol:
PDK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q16654
Protein GI Number:
3183120
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q16654
User NOte:
Optimal dilutions for each application to be determined by the researcher.