SP5 Antibody

ProSci
Product Code: PSI-64-195
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-195-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis of SP5 Antibody in mouse liver tissue lysates (35ug/lane)
2 / 2
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of SP5 Antibody in mouse liver tissue lysates (35ug/lane)
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Transcription factor Sp5, SP5
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
SP5 binds to GC boxes promoters elements. SP5 probable transcriptional activator that has a role in the coordination of changes in transcription required to generate pattern in the developing embryo.
Background References:
  • Chen, Y., et al. Biochem. Biophys. Res. Commun. 340(3):758-766(2006)
  • Suske, G., et al. Genomics 85(5):551-556(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-398 amino acids from the C-terminal region of human SP5.
NCBI Gene ID #:
389058
NCBI Official Name:
Transcription factor Sp5
NCBI Official Symbol:
SP5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q6BEB4
Protein GI Number:
74762296
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q6BEB4
User NOte:
Optimal dilutions for each application to be determined by the researcher.