TUBA1C Antibody

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ProSci
Product Code: PSI-61-048
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-048-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of MCF-7 cells using TUBA1C Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Western blot analysis of lysates from HT-1080, T47D cell line, mouse brain and testis tissue lysate (from left to right), using TUBA1C Antibody at 1:1000 at each lane.
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Flow cytometric analysis of MCF-7 cells using TUBA1C Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from HT-1080, T47D cell line, mouse brain and testis tissue lysate (from left to right), using TUBA1C Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Tubulin alpha-1C chain, Alpha-tubulin 6, Tubulin alpha-6 chain, TUBA1C, TUBA6
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Background References:
  • Lubec G.,et al.Submitted (MAR-2007) to UniProtKB.
  • Olsen J.V.,et al.Cell 127:635-648(2006).
  • Daub H.,et al.Mol. Cell 31:438-448(2008).
  • Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Hamster, Pig, Rat, Bovine, Monkey, Chicken, Drosophila, Xenopus
Immunogen:
This TUBA1C antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-60 amino acids from the N-terminal region of human TUBA1C.
NCBI Gene ID #:
84790
NCBI Official Name:
Tubulin alpha-1C chain
NCBI Official Symbol:
TUBA1C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q9BQE3
Protein GI Number:
20455322
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9BQE3
User NOte:
Optimal dilutions for each application to be determined by the researcher.