COL5A2 Antibody

ProSci
Product Code: PSI-62-851
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-851-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of COL5A2 Antibody in mouse lung tissue lysates (35ug/lane)
2 / 2
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of COL5A2 Antibody in mouse lung tissue lysates (35ug/lane)
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Collagen alpha-2(V) chain, COL5A2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
COL5A2 is an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This protein is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations.
Background References:
  • Menon,R., Reprod. Biol. Endocrinol.(1), 62 (2009)
  • Videman,T., Arthritis Rheum. 60 (2), 470-481 (2009)
  • Segev,F., Invest. Ophthalmol. Vis. Sci. 47 (2), 565-573 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL5A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the N-terminal region of human COL5A2.
NCBI Gene ID #:
1290
NCBI Official Name:
Collagen alpha-2(V) chain
NCBI Official Symbol:
COL5A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
145 kDa
Protein Accession #:
P05997
Protein GI Number:
143811378
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P05997
User NOte:
Optimal dilutions for each application to be determined by the researcher.